新型冠状病毒肺炎疫情期间肺癌患者的应对措施

新型冠状病毒肺炎(COVID-19)是近段时间我国面临的重大公卫事件，我国是肺癌大国，为了加强肺癌患者对这一疾病的认识，在疫情期间，使广大肺癌患者做好自身疾病的管理，做好对新型冠状病毒肺炎的预防。本文结合肺癌患者自身特点，对新型冠状病毒肺炎的易感性、鉴别诊断、防护、治疗，以及肺癌患者的随访、抗肿瘤用药、营养、手术等方面进行探讨，并结合两者的关系综合归纳，可以为广大肺癌患者更好地应对疫情提供参考。     

医用粘胶相关性皮肤损伤的文献计量学分析

目的: 应用文献计量学方法,分析国内外医用粘胶相关性皮肤损伤（Medical adhesive-related skin injuries,MARSI）文献的研究主题和热点。方法: 检索Cochrane Library、PubMed、EMbase、Web of science、sinoMed、CNKI、Wanfang、VIP等数据库MARSI相关文献,检索时限为建库至2019年5月。采用SPSS 22.0 软件进行文献计量分析,利用gCLUTO统计对国内外高频主题词进行聚类分析。结果: 共纳入论文103篇,其中外文文献占总文献量的27.18%,中文文献占总文献量的72.82%。聚类分析将国内外高频主题词聚为4大类,依次为皮肤损伤及损伤类型、相关危险因素、风险评估及风险管理、护理干预措施。结论: MARSI仍是国内外研究的热点,其发病机制研究仍处于探索阶段,目前尚缺乏有效的预防策略,研究领域较局限。在今后的研究中,应加强MARSI预防的研究、注重多学科合作。     

miR-145调控肝癌腹水模型Th9细胞升高的机制研究

目的:探讨miR-145调控肝癌腹水模型Th9细胞升高的作用机制。方法:构建小鼠H22肝癌腹水模型。造模两周后处死小鼠并分离出脾脏组织，流式细胞术分析肝癌腹水组（MA组）与正常对照组（Control 组）小鼠脾脏Th9细胞表达水平。ELISA法检测脾脏IL-9表达水平。RT-PCR法检测脾脏miR-145表达水平。Western Blot法检测脾脏中PI3K/Akt/mTOR/P70S6K/HIF-1α相关蛋白表达水平。分选小鼠脾脏CD4+T细胞，随机分为miR-145 mimics组和NC组，分别应用miR-145-5P mimics、阴性对照寡核苷酸（negative control，NC）进行转染，RT-PCR检测各组miR-145、HIF-1α mRNA及IL-9 mRNA表达水平。结果:与Control 组相比，MA组Th9细胞及其细胞因子IL-9表达均升高（P<0.05），miR-145表达降低（P<0.05），p-PI3K、p-Akt、mTOR、p-mTOR、p-P70S6K、HIF-1α蛋白均升高（P<0.05）。与NC组相比，miR-145 mimics组miR-145显著升高，HIF-1α mRNA及IL-9 mRNA表达下降。结论:肝癌腹水中Th9细胞升高可能与miR-145下降导致的PI3K/Akt/mTOR/P70S6K/HIF-1α通路激活有关。     

男性青少年性别角色冲突量表在中国青少年中的修订

目的:在中国青少年中修订男性青少年性别角色冲突量表。方法:以整群抽样法选取1230名男性青少年,对男性青少年性别角色冲突量表进行项目分析、探索性因素分析、验证性因素分析和信效度检验。结果:1量表包括28个条目;2探索性因素分析得出包括对成功和成就的需求、限制性情感表达、同性间限制性亲密行为和工作、学业与家庭的冲突、父母期待五个因子,共解释54.369%的方差变异;3修订后的量表信效度良好:总量表与各分量表的内部一致性系数为0.829、0.607、0.761、0.691、0.746、0.808,一个月后的重测信度分别为0.766、0.791、0.797、0.770、0.781、0.714,与自我和谐量表表现出良好的效标关联效度。结论:男性青少年性别角色冲突量表(中国青少年版)五因子结构稳定,各项心理学测量指标良好。     

Interleukin 17A genetic variations and susceptibility to non‐small cell lung cancer

Lung cancer is the leading cause of cancer‐related death, in which non‐small cell lung cancer (NSCLC) is the most common type. Evidence have shown that interleukin 17 (IL‐17) greatly involves in human immune responses. In this study, we investigated the effect of IL‐17 on NSCLC by examining the association between IL‐17A genetic polymorphisms and the susceptibility to NSCLC. IL‐17A ‐420A/G and IL‐17A ‐73G/A polymorphisms were detected in 330 NSCLC patients and 382 healthy controls. We found that subjects carrying ?73GA genotype or AA genotype had 2.09‐fold or 2.52‐fold increased risk of NSCLC than those with ?73GG genotype [odds ratio (OR) = 2.09, 95% confidence interval (CI), 1.46 – 2.98, p < 0.001; OR = 2.52, 95% CI, 1.30–4.88, p = 0.005, respectively). However, the IL‐17A ‐420A/G did not reveal any correlation with the cancer. Further investigation showed that prevalence of IL‐17A ?73GA genotype and A allele were significantly increased in adenocarcinoma patients (OR = 1.75, 95% CI, 1.08–2.86, p = 0.024, OR = 1.57, 95% CI, 1.09–2.28, p = 0.016, respectively). We also evaluated the effect of the polymorphisms on gene expression, and identified that peripheral blood mononuclear cells with IL‐17A ‐73GA and AA genotypes produced significantly higher level of IL‐17 than the cells with IL‐17A ‐73GG genotype. Our results suggest that IL‐17A ‐73G/A genetic variations may upregulate IL‐17 expression and are associated with increased susceptibility to NSCLC.     

Characterization of two pigeon paramyxovirus type 1 isolates in China

For over three decades, there has been a continuing panzootic caused by a virulent variant avian paramyxovirus type 1 strain, the so-called pigeon paramyxovirus type 1. It is found primarily in racing pigeons, but it has also spread to wild birds and poultry. In this study, two pigeon paramyxovirus type 1 strains, SD12 and BJ13, obtained from diseased pigeons in China, were characterized. Phylogenetic analysis based on complete sequences allowed characterization of both strains as genotype VI, class II. Further phylogenetic analysis of a 374-nucleotide section of the fusion gene showed that SD12 fell into lineage VIbii-d and BJ13 into VIbii-f. The deduced amino acid sequence of the cleavage site of the fusion protein confirmed that both isolates contained the virulent motif ¹¹²K/RRQKR↓F¹¹⁷ at the cleavage site. Nevertheless, the values of intracerebral pathogenicity indices showed the SD12 isolate to be a velogenic strain and BJ13 isolate to be a mesogenic strain. The SD12 isolate was further investigated via clinical observation, RNA detection, histopathology and viral serology in experimentally infected 3-week-old chickens. It showed a mild pathological phenotype in chickens, with viral replication restricted to a few tissues. The molecular mechanism for the SD12 isolate to have a virulent motif but low levels of virulence for chickens requires further study.